PEPTIDE CHEMISTRY (Gamme STRATOSPHERES™)

 

Resins for Peptide Synthesis

 

StratoSpheres resins for Peptide Synthesis are polystyrene functionalized resins for use in solid phase and solution phase peptide synthesis. For solid phase peptide synthesis, we have an extensive range of resins designed to generate peptide acids or peptide amides via an Fmoc or Boc synthetic strategy.

The latest addition to the StratoSpheres range are AmphiSpheres™ resins: amphipathic resins containing both polyethyleneglycol and polystyrene making them suitable for synthesis of “difficult” peptides on both small and large scale.

As with all StratoSpheres products, our resins for peptide synthesis offer the advantages of high quality, exceptional batch-to-batch reproducibility and tightly controlled, optimized particle sizes.

This in turn provides for improved product quality and increased yields. All products are available from lab scale to multi-kilogram scale.

Amph

iSpheres™ Resins for Peptide Synthesis

 

AmphiSpheres is a major new product introduction to the StratoSpheres range of products for solid phase peptide synthesis. AmphiSphere products are a family of resins produced using new Varian patent-pending chemistry that, as the name implies, are amphipathic (or amphiphilic) in nature; they have both hydrophobic and hydrophilic properties. This makes them excellent for synthesis of hard-to-make peptides and, importantly, AmphiSpheres products can supplied at large scale.

  • Applications
  • Products

> Amino Acid Loaded PL-CMS/ Boc-AA-CMS Resin

BOC-AA-CMS Resins 

Boc-Amino acids pre-loaded on PL-CMS Resin are designed for the synthesis of small to medium sized peptide acids.

Application Notes (PDF format)

> Amino Acid Loaded PL-MBHA/Boc-AA-MBHA resin

BOC-AA-MBHA Resins

 

Boc-Amino Acids pre-loaded on our PL-MBHA Resin.

Application Notes (PDF format)

> Amino Acid Loaded PL-Rink/ Fmoc-AA-Rink Resin

Fmoc-AA-Rink Resins

Fmoc-Amino Acids pre-loaded on our PL-Rink Resin are designed for the synthesis of peptide amides.

Application Notes (PDF Format)

> Amino Acid Loaded PL-Wang

Fmoc-AA-Wang Resins 

Fmoc-Amino Acids pre-loaded on our PL-Wang Resin are designed for the synthesis of peptide acids.

Application Notes (PDF Format)

> PL-AMS Resin 

PL-AMS is manufactured from Varian Polymer Lab's PL-CMS resin which enables us to utilize the same degree of control over loading levels for the hugely popular chloromethyl polystyrene resin, with the assured quality this offers.

Application Notes (PDF Format)

> PL Cl-TRT-Cl Resin

PL Cl-Trt-Cl Resin is particularly suited to the generation of protected peptide fragments due to the extremely mild cleavage conditions required. Such resins can also be used for synthesis of alcohols, phenols and amines.

Application Notes (PDF Format)

> PL-CMS Resin 

PL-CMS Resin is a contemporary polystyrene-based resin manufactured by a proprietary copolymerization technique. This eliminates the need for Friedel-Crafts functionalization and provides a homogeneous polymer of extremely high purity and optimum reproducibility.

Merrifield Peptide Synthesis

PL-CMS Resin is available in a variety of loadings ranging from 0.4 mmol/g to 1.4 mmol/g (in 0.2 mmol/g increments) with 75-150 µm (100-200 mesh) particle size for Merrifield peptide synthesis techniques. Particle size distribution is narrower than other comparable commercially available resins, and the beads possess a high degree of uniformity. The range of loadings allows for synthesis optimization - larger peptides are generally prepared on lower loading resin; smaller peptides are made more efficiently on higher loading resins.

Combinatorial Synthesis

Varian Polymer Labs has developed the StratoSpheres range of products to meet the demands of combinatorial synthesis, achieving significant increases in loading per bead for optimum performance from small molecule libraries. Using our PL-CMS resin, screening and sequencing can now be reliably achieved from a single bead. High load PL-CMS resins are available in 2.0 mmol/g and 4.0 mmol/g loading in narrow particle size distributions up to 500 µm.

Application Notes (PDF Format)

> PL-DMA Resin 

PL-DMA resin is generally used for solid phase peptide synthesis using (but not restricted to) Fmoc protection strategy. Commonly known as Sheppard’s polyamide resin, it was developed from the concept that the polymer support and peptide chain should possess similar polarities and solvation properties. Unlike the polystyrene-based supports, the PL-DMA resin possesses high swell characteristics in N,N-dimethylformamide and other polar aprotic solvents.

Application Notes (PDF Format)

> PL-HMBA Resin

PL-HMBA Resin is used for attachment of carboxylic acids, including amino acids. The stability of the ester bond allows acidolysis to be used to cleave side chain protecting groups while the product is still bound to the resin. The ester bond is labile to nucleophiles and so ammonolysis or saponification can be used to generate amides or acids respectively.

Application Notes (PDF Format)

> PL-MBHA Resin 

PL-MBHA Resin is for the synthesis of amides.

Traditional support for solid phase synthesis of peptide amides using Boc chemistry.

Initial amino acids can be coupled directly to the resin using standard coupling techniques.

Cleavage typically requires treatment with very strong acid such as HF or TFMSA. Note: Specialist equipment is required to safely perform HF cleavage operations.

Sometimes Fmoc peptide synthesis is performed on PL-MBHA Resin to allow for removal of side chain protecting groups while the peptide chain remains attached to the resin. HF cleavage then ensures that only the desired unprotected peptide amide is released into solution, free from contamination with cleaved side chain protecting groups.

PL-MBHA Resin can also be used for attachment of linkers in a similar manner to PL-AMS Resin.

Application Notes (PDF Format)

> PL-Oxime Resin

PL-Oxime Resin is designed for the attachment of acids via anhydride activation methods. Displacement by hydrazinolysis, ammonolysis or hydrolysis results in a variety of functional group conversions. Oxime resins have also been used in the synthesis of ureas, hydroxamic acids, piperazinediones and benzisoxazoles.

Application Notes (PDF Format)

> PL-PEGA Resin

PL-PEGA Resin is a unique polyamide/polyethylene glycol copolymer. This truly hydrophilic copolymer system provides a greater and more uniform expansion across a wide range of solvents than its PEG grafted polystyrene counterpart.

PL-PEGA is sold under license from Carlsberg Laboratory.

Application Notes (PDF Format)

> PL-Ramage Resin 



Ramage resin is particularly acid sensitive and is therefore suitable for the preparation of fully protected peptide amides via Fmoc chemistry.

Peptide cleavage can be accomplished by treatment with just 2-3% TFA. The resultant fully protected peptide amide can then be purified before use in a solution phase fragment condensation reaction, or it can be treated with a higher concentration of acid in order to remove any side chain protecting groups while in solution.

Application Notes (PDF Format)

> PL-Rink

PL-Rink Resin is typically used for the manufacture of peptide amides, although combinatorial approaches for generation of amides and sulfonamides are also appropriate. The resin is comprised of the Fmoc-protected Rink linker attached directly to a high quality PL-AMS resin. Following deprotection, carboxylic acids may be loaded onto the resultant primary amine group. The corresponding carboxamide can be released by simple treatment with trifluoroacetic acid (TFA).

PL-Rink Resin is sold under license from Aventis.

Application Notes (PDF Format)

> PL-Rink MBHA 

PL-Rink MBHA Resin is widely used for the solid phase synthesis of peptide amides using Fmoc chemistry. It is designed to allow the attachment of carboxylic acids, which are subsequently cleaved as amides.

PL-Rink Resin is sold under license from Aventis.

Application Notes (PDF Format)

> PL-Sieber Resin 

This xanthenyl-based resin may be cleaved using very low concentrations of TFA (1 – 2%), thereby allowing peptide amides to be produced with their protecting groups intact. This allows the possibility of purifying the resultant peptide amide prior to fragment assembly, and subsequently removing the remaining side chain protecting groups under optimized solution phase conditions.

Using PL-Sieber Resin, it is possible to utilize a C-terminal protected aspartic acid or glutamic acid and attach to the resin via the carboxylic acid side chain. On acidolysis, the corresponding asparagine or glutamine species is generated allowing the synthesis of peptides containing C-terminal esters of Asn or Gln, or cyclic peptides (should a suitable orthogonal protecting group be used).

Application Notes (PDF Format)

> PL-Wang

PL-Wang is an alkoxybenzylalcohol derivative manufactured from our own PL-CMS resin. This product is designed for acid labile cleavage (using 95% TFA) and is ideally suited to Fmoc protection synthesis strategies.

Application Notes (PDF Format)

> PL-Weinreb

PL-Weinreb Resin is produced by attachment of N-Fmoc-N-methoxy-ß-alanine to PL-AMS Resin. Removal of the Fmoc protecting group with piperidine solution is required before attachment of a suitable carboxylic acid to the methoxylamine. This reaction can prove sluggish due to the steric interference of the methoxy group. The resulting Weinreb amide equivalent will generate aldehydes by treatment with LiAlH4.

Application Notes (PDF Format)